melatonin improves development of early mouse embryos impaired by actinomycin-d and tnf-α

Melatonin improves development of early mouse embryos impaired by actinomycin-D and TNF-α

BACKGROUND Melatonin, a reactive oxygen species (ROS) scavenger and an antioxidant, has been shown that can inhibit apoptosis. Administration of melatonin may improve embryo development in assisted reproductive technology (ART). OBJECTIVE The aim of this study was to evaluate the role of melatonin in inhibition of spontaneous and induced apoptosis by Tumor Necrosis Factor Alph (TNF-α) and act...

The effects of actinomycin D on the early development of quail and chick embryos.

In agreement with previous work it has been found that treatment of chick embryos at an early stage of development with small concentrations of actinomycin D (20-40 x 10~ M) produces abnormalities of the brain, neural tube and somites but allows near normal development of heart and blood islands. Similar effects are produced on quail embryos by even smaller concentrations of actinomycin D (0-8-...

The Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos

Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent...

The development of mouse early embryos in vitro in fibroblasts and cumulus cells co-cultures supplemented with retinoic acid

This study was designed to examine the effects of retinoic acid adding to cumulus and/or fibroblast cellsmonolayer on the development of mouse early embryos. One-cell mouse embryos were obtained from NMRImice after superovulation by an intraperitoneal injection of 5 IU equine chorionic gonadotrophin (eCG)followed 48 hrs later by 5 IU human chorionic gonadotrophin (hCG). Mouse embryonic fibrobla...

TERATOGENIC EFFECTS OF LITHIUM CARBONATE ON DEVELOPMENT OF MOUSE EMBRYOS

Development of early mouse and rabbit embryos without zona pellucida.

Criteria for assessing embryo vitality of denuded rabbit and mouse embryos in vitro were: volume of the embryo, number of cells and number of metaphase plates after 24 and 48 h in culture. Mouse embryos grew normally, with and without the zona pellucida, but denuded rabbit embryos showed reduced growth (volume and cell number) and failure to implant in synchronized recipients.